Bartholomew played college football for the Tennessee Volunteers football team, earning many honors including 2001 SEC Good Works Team, Verizon Academic All-District IV, Academic All-SEC; 2000 Academic All-SEC; 1999 Academic All-SEC; and 1998 Academic All-SEC. He functioned primarily as a "blocking" back for the team.
As an undrafted free agent, Bartholomew was to play in the National Football League for the Denver Broncos. Bartholomew's career ended after suffering a knee injury during training camp that required multiple extensive surgeries.
Bartholomew is the founder & CEO of D1 Sports, a training and therapy company dedicated to being The PLACE for the ATHLETE. 
Bartholomew's brother, Ben, also played for the Tennessee Volunteers.
- "Will Bartholomew: All in the Family". University of Tennessee. October 3, 2001. http://www.utsports.com/sports/m-footbl/spec-rel/100301aaa.html. Retrieved January 3, 2011.
- "Will Bartholomew, FB - Tennessee". USA Today. April 19, 2001. https://www.usatoday.com/sports/nfl/2002draft/fbs/willbartholomew.htm. Retrieved January 3, 2011.
- Lawley, Erin (April 2, 2006). "D1 Sports Training hits upon winning strategy for growth". Nashville Business Journal. http://www.bizjournals.com/nashville/stories/2006/04/03/story8.html. Retrieved January 3, 2011.
- "Ben Bartholomew". utsports.com. http://www.utsports.com/sports/m-footbl/mtt/bartholomew_ben00.html. Retrieved March 4, 2016.