Biolo was born on February 8, 1916 in Iron Mountain, Michigan. John died on February 4, 2003 in Fond du Lac, Wisconsin. He was the great uncle to percussionist Carrie Biolo.
John attended Lake Forest College (1935–38) and lettered in baseball and football. John captained the undefeated 1938 football team and was named a Little All American. John is all enshrined in the Lake Forest Athletic Hall of Fame. Biolo was a member of the Green Bay Packers during the 1939 NFL season. As such, he was a member of the 1939 NFL Champion Packers.
John continued on as a Player / Coach with the Kenosha Cardinals in 1940 and 41 of the American Pro Football League. John joined the US Navy in 1941 and was commissioned an officer. John flew anti-sub patrols in the canal zone and then received carrier qualification in fighter - bombers in preparation for the projected invasion of Japan. John attained the rank of Lieutenant.
After the war, John taught and coached at St. Norbert College in De Pere, Wisconsin.
In 1951 he moved to West High School in Green Bay, Wisconsin and served as assistant principal and Head Football Coach, leading his teams to four straight undefeated seasons. Named the Fox Valley Conference Coach of the Year five times, he led several teams to the Wisconsin Championships in football and track and eventually became principal of the school. He was selected to the All Upper Peninsula Hall of Fame and the Wisconsin State Coaches Hall of Fame. Elected to six years on the WIAA Advisory Council, which oversees high school athletics in Wisconsin, he also served as president of the council.
- List of Green Bay Packers players
- NFL champion (1939)