After two years with the Washington Redskins in 1957-58, Dee returned to Holy Cross to tutor the team's linemen.
He became an ironman of the American Football League who never missed a game during his career, starting 112 consecutive games. Despite equipment improvements over the years, Dee was a superstitious player who chose to wear the same helmet throughout his career (105 of 112 games). Dee etched his name in the history books by scoring the first points in American Football League history, scoring a touchdown when he dove onto a fumble by Bills QB Tommy O'Connell (Father of former Boston Bruins GM Mike O'Connell) the end zone in the second quarter of the league's first-ever exhibition game, a contest between the Patriots and the Buffalo on July 30, 1960. He was voted to four American Football League All-Star teams (1961, 1963–65) and is a member of the Patriots All-1960s (AFL) Team.
Bob Dee recorded 33 QB sacks (not including his strip sack of Tommy O'Connell in the AFL's first Exhibition Game).
Bob sacked Frank Tripucka, Al Dorow, Hunter Enis, Jacky Lee, MC Reynolds, Randy Duncan, Cotton Davidson, George Blanda, Jack Kemp, Johnny Green, John Hadl, Tobin Rote, Len Dawson, Eddie Wilson, Dick Wood, Joe Namath, Tom Flores, Rick Norton and Bob Griese and recovered fumbles by Al Carmichael, Art Baker, Wayne Crow, Jacky Lee, Paul Lowe, Bill Tobin, Wray Carlton & Max Chobian.
He had two interceptions in the Patriots 26-8 Eastern Divisional Playoff Game win over the Buffalo Bills because he was wearing one sneaker and one football shoe with spikes so was he able to maneuver better in the snow in the game played at War Memorial Stadium on 12-28-63.
On July 22, 1968, Dee announced his retirement from professional football, citing a business opportunity that was "too good to resist."
He was awarded a game ball for his outstanding performance in the Patriots 34-17 win over the Houston Oilers on 11-29-64.
He was inducted in the Patriots Hall of Fame on August 18, 1993.
In recognition of his accomplishments on the field, the Patriots retired his number (89).