1900 Iowa State Cyclones football team

The 1900 Iowa State Cyclones football team represented Iowa State College of Agricultural and Mechanic Arts (later renamed Iowa State University) as an independent during the 1900 college football season. In their first and only season under head coach C. E. Woodruff, the Cyclones compiled a 2–5–1 record and were outscored by opponents by a combined total of 100 to 38. L. M. Chambers was the team captain.

Between 1892 and 1913, the football team played on a field that later became the site of the university's Parks Library.