1916 Iowa State Cyclones football team

The 1916 Iowa State Cyclones football team represented Iowa State College of Agricultural and Mechanic Arts (later renamed Iowa State University) in the Missouri Valley Conference during the 1916 college football season. In their second season under head coach Charles Mayser, the Cyclones compiled a 5–2–1 record (2–1–1 against conference opponents), finished in third place in the conference, shut out five of eight opponents, and outscored opponents by a combined total of 107 to 36. They played their home games at State Field in Ames, Iowa. Dury Moss was the team captain.